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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
12/12/2017 |
Data da última atualização: |
10/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SIQUEIRA, L. G. B.; TRIBULO, P.; CHEN, Z.; DENICOL, A. C.; ORTEGA, M. S.; NEGRON-PEREZ, V.M.; KANNAMPUZHA-FRANCIS, J. P.; POHLER, K. G.; RIVERA, R. M.; HANSEN, P. J. |
Afiliação: |
LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; P. TRIBULO, UNIVERSITY OF FLORIDA/USA; Zhiyuan Chen, University of Missouri, Columbia, MO; A. C. DENICOL, UNIVERSITY OF FLORIDA/USA; M. S. ORTEGA, UNIVERSITY OF FLORIDA/USA; V. M. NEGRON-PEREZ, UNIVERSITY OF FLORIDA/USA; J. P. KANNAMPUZHA-FRANCIS, UNIVERSITY OF FLORIDA/USA; Ky G. Pohler, University of Tennessee, Knoxville, TN; Rocio M. Rivera, University of Missouri, Columbia, MO; P. J. HANSEN, UNIVERSITY OF FLORIDA/USA. |
Título: |
Colony-stimulating factor 2 acts from days 5 to 7 of development to modify programming of the bovine conceptus at day 86 of gestation. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Biology of Reproduction, v. 96, n. 4, p. 743-757, 2017. |
DOI: |
http://doi.org/10.1093/biolre/iox018 |
Idioma: |
Inglês |
Conteúdo: |
Colony-stimulating factor 2 (CSF2) is an embryokine that improves competence of the embryo to establish pregnancy and which may participate in developmental programming. We tested whether culture of bovine embryos with CSF2 alters fetal development and alleviates abnormalities associated with in vitro production (IVP) of embryos. Pregnancies were established by artificial insemination (AI), transfer of an IVP embryo (IVP) or transfer of an IVP embryo treated with 10 ng/ml CSF2 from day 5 to 7 of development (CSF2). Pregnancies were produced using X-sorted semen from a single sire. Female singleton conceptuses were collected on day 86 of gestation. There were few morphological differences between groups although IVP and CSF2 fetuses were heavier than AI fetuses. Bicarbonate concentration in allantoic fluid was lower for IVP than for AI or CSF2. Expression of 92 genes in liver, placenta, and muscle was determined by RT-qPCR. The general pattern for liver and placenta was for IVP to alter expression and for CSF2 to sometimes reverse this effect. For muscle, CSF2 affected gene expression but did not generally reverse effects of IVP. Levels of methylation for each of the three tissues at 12 loci in the promoter of IGF2 and five in the promoter of GRB10 were unaffected by treatment except for CSF2 effects on two CpG for IGF2 in placenta and muscle. In conclusion, CSF2 can act as a developmental programming agent but alone is not able to abolish the adverse effects of in vitro production on fetal characteristics. MenosColony-stimulating factor 2 (CSF2) is an embryokine that improves competence of the embryo to establish pregnancy and which may participate in developmental programming. We tested whether culture of bovine embryos with CSF2 alters fetal development and alleviates abnormalities associated with in vitro production (IVP) of embryos. Pregnancies were established by artificial insemination (AI), transfer of an IVP embryo (IVP) or transfer of an IVP embryo treated with 10 ng/ml CSF2 from day 5 to 7 of development (CSF2). Pregnancies were produced using X-sorted semen from a single sire. Female singleton conceptuses were collected on day 86 of gestation. There were few morphological differences between groups although IVP and CSF2 fetuses were heavier than AI fetuses. Bicarbonate concentration in allantoic fluid was lower for IVP than for AI or CSF2. Expression of 92 genes in liver, placenta, and muscle was determined by RT-qPCR. The general pattern for liver and placenta was for IVP to alter expression and for CSF2 to sometimes reverse this effect. For muscle, CSF2 affected gene expression but did not generally reverse effects of IVP. Levels of methylation for each of the three tissues at 12 loci in the promoter of IGF2 and five in the promoter of GRB10 were unaffected by treatment except for CSF2 effects on two CpG for IGF2 in placenta and muscle. In conclusion, CSF2 can act as a developmental programming agent but alone is not able to abolish the adverse effects of in vitro prod... Mostrar Tudo |
Palavras-Chave: |
Colony-stimulating factor 2; Embryokine; Programming. |
Thesaurus Nal: |
fetal development. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02445naa a2200289 a 4500 001 2082306 005 2022-08-10 008 2017 bl uuuu u00u1 u #d 024 7 $ahttp://doi.org/10.1093/biolre/iox018$2DOI 100 1 $aSIQUEIRA, L. G. B. 245 $aColony-stimulating factor 2 acts from days 5 to 7 of development to modify programming of the bovine conceptus at day 86 of gestation.$h[electronic resource] 260 $c2017 520 $aColony-stimulating factor 2 (CSF2) is an embryokine that improves competence of the embryo to establish pregnancy and which may participate in developmental programming. We tested whether culture of bovine embryos with CSF2 alters fetal development and alleviates abnormalities associated with in vitro production (IVP) of embryos. Pregnancies were established by artificial insemination (AI), transfer of an IVP embryo (IVP) or transfer of an IVP embryo treated with 10 ng/ml CSF2 from day 5 to 7 of development (CSF2). Pregnancies were produced using X-sorted semen from a single sire. Female singleton conceptuses were collected on day 86 of gestation. There were few morphological differences between groups although IVP and CSF2 fetuses were heavier than AI fetuses. Bicarbonate concentration in allantoic fluid was lower for IVP than for AI or CSF2. Expression of 92 genes in liver, placenta, and muscle was determined by RT-qPCR. The general pattern for liver and placenta was for IVP to alter expression and for CSF2 to sometimes reverse this effect. For muscle, CSF2 affected gene expression but did not generally reverse effects of IVP. Levels of methylation for each of the three tissues at 12 loci in the promoter of IGF2 and five in the promoter of GRB10 were unaffected by treatment except for CSF2 effects on two CpG for IGF2 in placenta and muscle. In conclusion, CSF2 can act as a developmental programming agent but alone is not able to abolish the adverse effects of in vitro production on fetal characteristics. 650 $afetal development 653 $aColony-stimulating factor 2 653 $aEmbryokine 653 $aProgramming 700 1 $aTRIBULO, P. 700 1 $aCHEN, Z. 700 1 $aDENICOL, A. C. 700 1 $aORTEGA, M. S. 700 1 $aNEGRON-PEREZ, V.M. 700 1 $aKANNAMPUZHA-FRANCIS, J. P. 700 1 $aPOHLER, K. G. 700 1 $aRIVERA, R. M. 700 1 $aHANSEN, P. J. 773 $tBiology of Reproduction$gv. 96, n. 4, p. 743-757, 2017.
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| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
12/05/2021 |
Data da última atualização: |
12/05/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
VIEIRA, T. F.; CORRÊA, R. C. G.; MOREIRA, R. de F. P. M.; PERALTA, R. A.; LIMA, E. A. de; HELM, C. V.; GARCIA, J. A. A.; BRACHT, A.; PERALTA, R. M. |
Afiliação: |
TATIANE FRANCIELLI VIEIRA, Universidade Estadual de Maringá; RÚBIA CARVALHO GOMES CORRÊA, UniCesumar; REGINA DE FATIMA PERALTA MUNIZ MOREIRA, Universidade Federal de Santa Catarina; ROSELY APARECIDA PERALTA, Universidade Federal de Santa Catarina; EDSON ALVES DE LIMA, CNPF; CRISTIANE VIEIRA HELM, CNPF; JÉSSICA AMANDA ANDRADE GARCIA, Universidade Estadual de Maringá; ADELAR BRACHT, Universidade Estadual de Maringá; ROSANE M. PERALTA, Universidade Estadual de Maringá. |
Título: |
Valorization of peach palm (Bactris gasipaes Kunth) waste: production of antioxidant xylooligosaccharides. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Waste and Biomass Valorization, 2021. |
DOI: |
https://doi.org/10.1007/s12649-021-01457-3 |
Idioma: |
Português |
Notas: |
Publicado em 6 de maio de 2021. |
Conteúdo: |
In Brazil, the production and consumption of palm heart, especially from the Bactris gasipaes Kunth, generates a large number of lignocellulosic by-products. This study reports the obtainment of xylooligosaccharides (XOS) from xylans extracted from these residues. Xylans from peach palm waste (inner sheath and peel) were extracted using a mild alkali treatment with recovery yields of 82% and 80%, respectively. XOS were obtained through enzymatic hydrolysis employing a commercial xylanase with yields from xylan inner sheath and xylan peel of 50.1% and 48.8%, respectively. The antioxidant potential of XOS was measured employing five of the most commonly used procedures. In overall terms, the XOS from the xylans of peach palm wastes showed higher antioxidant capacity than the XOS obtained from the commercial xylans. The chemical structures of the XOS were determined by mass spectrometry (ESI?MS). The ESI?MS spectra suggest that XOS with grouped xylose or arabinose units ranging from 2 to 5 (differing by 132 Da) and as sodium adduct ions [M + Na]+ in the range of 100?1000 m/z. These results indicate that peach palm wastes can be explored to XOS production, which could be applied as natural antioxidants in functional food and pharmaceutical preparations. |
Palavras-Chave: |
Produtos não madereiros; Resíduos de pupunheira; Xilanases; Xilooligossacarídeos. |
Thesagro: |
Antioxidante. |
Thesaurus NAL: |
Antioxidants; Xylanases; Xylooligosaccharides. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02304naa a2200337 a 4500 001 2131783 005 2021-05-12 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s12649-021-01457-3$2DOI 100 1 $aVIEIRA, T. F. 245 $aValorization of peach palm (Bactris gasipaes Kunth) waste$bproduction of antioxidant xylooligosaccharides.$h[electronic resource] 260 $c2021 500 $aPublicado em 6 de maio de 2021. 520 $aIn Brazil, the production and consumption of palm heart, especially from the Bactris gasipaes Kunth, generates a large number of lignocellulosic by-products. This study reports the obtainment of xylooligosaccharides (XOS) from xylans extracted from these residues. Xylans from peach palm waste (inner sheath and peel) were extracted using a mild alkali treatment with recovery yields of 82% and 80%, respectively. XOS were obtained through enzymatic hydrolysis employing a commercial xylanase with yields from xylan inner sheath and xylan peel of 50.1% and 48.8%, respectively. The antioxidant potential of XOS was measured employing five of the most commonly used procedures. In overall terms, the XOS from the xylans of peach palm wastes showed higher antioxidant capacity than the XOS obtained from the commercial xylans. The chemical structures of the XOS were determined by mass spectrometry (ESI?MS). The ESI?MS spectra suggest that XOS with grouped xylose or arabinose units ranging from 2 to 5 (differing by 132 Da) and as sodium adduct ions [M + Na]+ in the range of 100?1000 m/z. These results indicate that peach palm wastes can be explored to XOS production, which could be applied as natural antioxidants in functional food and pharmaceutical preparations. 650 $aAntioxidants 650 $aXylanases 650 $aXylooligosaccharides 650 $aAntioxidante 653 $aProdutos não madereiros 653 $aResíduos de pupunheira 653 $aXilanases 653 $aXilooligossacarídeos 700 1 $aCORRÊA, R. C. G. 700 1 $aMOREIRA, R. de F. P. M. 700 1 $aPERALTA, R. A. 700 1 $aLIMA, E. A. de 700 1 $aHELM, C. V. 700 1 $aGARCIA, J. A. A. 700 1 $aBRACHT, A. 700 1 $aPERALTA, R. M. 773 $tWaste and Biomass Valorization, 2021.
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